control system

Compared to DNA-sequence analysis modern DNA-fragment analysis needs several special features for reliable genotyping. Measured fragment data can not be used like sequence data without further processing. The processing comprises:

samples with allelic ladders, positive and negative controls.

Using a file name descriptor DNA-Match will recognise allelic ladders and control samples from the file name itself. Known and questioned samples are divided in two subgroups inside DNA-Match (control system and fragment analysis). Questioned samples are also identified by the file names (ABI-format) and the electropherograms are visualised and processed in the fragment analysis part.

Various sets of microsatellite markers with the alleles' expected bp values can be saved in a database.

Several sophisticated functions programmed in an expert system assist the assignment of peaks from allelic ladders.

Two or more different colours are used to display bins for present fragments/peaks (pink) and absent but possible peaks (grey). Expected peaks in a positive control have pink coloured bins (vertical bar).
A peak on a wrong position in a positive control therefore is easily rerecognised.